Sleator lab identifies single point mutation in Listeria monocytogenes

Public release date: 12-Mar-2013
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Contact: Andrew Thompson
andrew@landesbioscience.com
Landes Bioscience

The bacterial foodborne pathogen, Listeria monocytogenes is the causative agent of listeriosisa debilitating disease linked with ~2,500 illnesses and more than 500 deaths per annum in the US alone. A characteristic feature of L. monocytogenes is its ability to grow at refrigeration temperatures and in the presence of high concentrations of salttraditional food preservation techniques, which arrest the growth of most other pathogens.

Work in the Sleator lab has shown that the bacterium protects itself from such stresses by twisting into a protective corkscrew type shape in an effort to reduce its exposure to the stressin the same way a human might wrap up tighthugging the core to reduce the effects of the cold.

Furthermore, Sleator and colleagues have identified a single point mutation (out of a total of 3 million or so nucleotides that constitute the entire listerial genome), which dramatically improves the growth of the pathogen in the refrigerator.

The research paper, "A single point mutation in the listerial betL ?A-dependent promoter leads to improved osmo- and chill-tolerance and a morphological shift at elevated osmolarity," will be published in the November/December 2013 issue of Bioengineered. It is available open access ahead of press: http://www.landesbioscience.com/journals/bioe/article/24094/

Sleator claims that this mutation represents "a double edged sword;" "from a food safety perspective, a single point mutation with the potential to induce such dramatic shifts in cell growth and survival at low temperaturesmaking an already dangerous pathogen even more formidableraises significant food-safety concerns which need to be addressed." However, from a synthetic biology point of view, such a boosted-stress resistance gene represents a useful BioBrick (or building block) for the design of more physiologically robust probiotics or, indeed, plants that are more resistant to cold arid conditions.

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Published by Landes Bioscience since 2010, Bioengineered publishes relevant and high-impact original research with a special focus on genetic engineering that involves the generation of recombinant strains and their metabolic products for beneficial applications in food, medicine, industry, environment and bio-defense. Established in 2002, Landes Bioscience is an Austin, Texas-based publisher of biology research journals and books. For more information on Landes Bioscience, please visit http://www.landesbioscience.com/.

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Public release date: 12-Mar-2013
[ | E-mail | Share ]

Contact: Andrew Thompson
andrew@landesbioscience.com
Landes Bioscience

The bacterial foodborne pathogen, Listeria monocytogenes is the causative agent of listeriosisa debilitating disease linked with ~2,500 illnesses and more than 500 deaths per annum in the US alone. A characteristic feature of L. monocytogenes is its ability to grow at refrigeration temperatures and in the presence of high concentrations of salttraditional food preservation techniques, which arrest the growth of most other pathogens.

Work in the Sleator lab has shown that the bacterium protects itself from such stresses by twisting into a protective corkscrew type shape in an effort to reduce its exposure to the stressin the same way a human might wrap up tighthugging the core to reduce the effects of the cold.

Furthermore, Sleator and colleagues have identified a single point mutation (out of a total of 3 million or so nucleotides that constitute the entire listerial genome), which dramatically improves the growth of the pathogen in the refrigerator.

The research paper, "A single point mutation in the listerial betL ?A-dependent promoter leads to improved osmo- and chill-tolerance and a morphological shift at elevated osmolarity," will be published in the November/December 2013 issue of Bioengineered. It is available open access ahead of press: http://www.landesbioscience.com/journals/bioe/article/24094/

Sleator claims that this mutation represents "a double edged sword;" "from a food safety perspective, a single point mutation with the potential to induce such dramatic shifts in cell growth and survival at low temperaturesmaking an already dangerous pathogen even more formidableraises significant food-safety concerns which need to be addressed." However, from a synthetic biology point of view, such a boosted-stress resistance gene represents a useful BioBrick (or building block) for the design of more physiologically robust probiotics or, indeed, plants that are more resistant to cold arid conditions.

###

Published by Landes Bioscience since 2010, Bioengineered publishes relevant and high-impact original research with a special focus on genetic engineering that involves the generation of recombinant strains and their metabolic products for beneficial applications in food, medicine, industry, environment and bio-defense. Established in 2002, Landes Bioscience is an Austin, Texas-based publisher of biology research journals and books. For more information on Landes Bioscience, please visit http://www.landesbioscience.com/.

---

[ | E-mail | Share ]

?

AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.

Source: http://www.eurekalert.org/pub_releases/2013-03/lb-sli031213.php

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